Actin Antibody Cocktail (pan muscle)

• Catalog Number: orb749985
• Category: Antibodies
• Description: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HHF35 + MSA/953
• Tested applications: IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human actin fragment was used as the immunogen for the pan Muscle Actin antibody cocktail.
• Dilution range: Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This pan Muscle Actin antibody cocktail is available for research use only.
• UniProt ID: P62736, P68032, P68133
• Storage: Store the pan Muscle Actin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the pan Muscle Actin antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with pan Muscle Actin antibody (HHF35 + MSA/953)

IHC: Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with pan Muscle Actin antibody

IHC: Formalin-fixed, paraffin-embedded rat heart stained with pan Muscle Actin antibody

IHC: Formalin-fixed, paraffin-embedded rat stomach stained with pan Muscle Actin antibody

IHC: Formalin-fixed, paraffin-embedded rat lung stained with pan Muscle Actin antibody

SDS-PAGE analysis of purified, BSA-free pan Muscle Actin antibody cocktail antibody (clone HHF35 + MSA/953) as confirmation of integrity and purity.