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Catalog Number | orb2296444 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant ACTB. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3G4-F9 |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG1 Kappa |
Immunogen | ACTB (AAH01301, 1 a.a. ~ 375 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | MDDDIAALVVDNGSGMCKAGFAGDDAPRAVSPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERRYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF |
NCBI | AAH01301 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ACTB monoclonal antibody (M01), clone S1. Western Blot analysis of ACTB expression in K-562.
ACTB monoclonal antibody (M01), clone 3G4-F9 Western Blot analysis of ACTB expression in HeLa.
ACTB monoclonal antibody (M01), clone 3G4-F9. Western Blot analysis of ACTB expression in Raw 264.7.
ACTB monoclonal antibody (M01), clone 3G4-F9. Western Blot analysis of ACTB expression in NIH/3T3.
Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody (M01), clone 3G4-F9. Lane 1: ACTB transfected lysate(42 KDa). Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged ACTB is approximately 1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (66.99 KDa).