ACSS2 antibody

• Catalog Number: orb377893
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ACSS2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human ACAS2
• Dilution range: WB: 1:500-2000, IF/ICC: 1:10-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ACSS2
• Entrez: 55902, 60525
• UniProt ID: Q9QXG4, Q9NR19
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: ACAS2; Acetyl-coenzyme A synthetase, cytoplasmic;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ACAS2 expression in HepG2 (A), SW620 (B), mouse liver (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 78; 80 kD; Observed band size: 78 kD)

Immunofluorescent analysis of ACAS2 staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.