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Catalog Number | orb1474379 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to ACLY |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | FC, IF, WB |
Reactivity | Human, Mouse, Primate |
Immunogen | Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli. |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), FC (1/50 - 1/100) |
Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Conjugation | Unconjugated |
Target | ACLY |
Entrez | 47, 104112 |
UniProt ID | Q91V92, P53396 |
Source | Mouse |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Alternative names | ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ACLY expression in NIH3T3 (A), K562 (B), COS7 (C), Hela (D) whole cell lysates. (Predicted band size: 121 kD; Observed band size: 121 kD)
Immunofluorescent analysis of ACLY staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Flow cytometric analysis of ACLY in HeLa cells (red). Blue line histogram represents the isotype control.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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