Acid Phosphatase antibody (Peroxidase)

• Catalog Number: orb344866
• Category: Antibodies
• Description: Acid Phosphatase antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Acid Phosphatase [Potato]
• Concentration: 1.0 mg/mL
• Dilution range: WB: 1:250
• Form/Appearance: Liquid (sterile filtered)
• Purity: Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: Q6J5M8
• NCBI: AAT37528.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: rabbit anti-Acid Phosphatase Antibody Peroxidase C
• Note: For research use only
• Application notes: Anti-Acid Phosphatase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Acid Phosphatase (Potato) Antibody. Lane 1: Acid Phosphatase (Potato). Load: 50 ng per lane. Primary antibody: Acid Phosphatase Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 49 kDa/42-45 kDa for Acid Phosphatase (Potato) Protein. Other band(s): Acid Phosphatase monomer fragmentation.