Acid Phosphatase antibody (Biotin)

• Catalog Number: orb344938
• Category: Antibodies
• Description: Acid Phosphatase antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Acid Phosphatase [Potato]
• Concentration: 1.0 mg/mL
• Dilution range: WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other sources is unknown.
• Conjugation: Biotin
• UniProt ID: Q6J5M8
• NCBI: AAT37528.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Acid Phosphatase Antibody biotin Conju
• Note: For research use only
• Application notes: Anti-Acid Phosphatase Biotin Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rabbit Anti-Acid Phosphatase Biotin Conjugated. Antigen: Acid Phosphatase. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Acid Phosphatase Biotin 10 µg/mL for 1 hr at RT. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT.