Acetylated Lysine Antibody: HRP

• Catalog Number: orb147383
• Category: Antibodies
• Description: Mouse monoclonal to Acetylated Lysine (HRP). Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Protein posttranslational reversible lysine Ne development..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7F8
• Tested applications: ELISA, IHC, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Acetylated KLH
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100)
• Conjugation: HRP
• Target: Acetylated Lysine
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: Acetyl Lysine antibody, Acetylated lysine antibody
• Note: For research use only
• Application notes: 1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.

Western Blot analysis of acetylated lysine showing detection of Acetylated Lysine protein using Mouse Anti-Acetylated Lysine Monoclonal Antibody, Clone 7F8. Primary Antibody: Mouse Anti-Acetylated Lysine Monoclonal Antibody at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.