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Catalog Number | orb699633 |
---|---|
Category | Antibodies |
Description | This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ACE/3765 |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | A portion of amino acids 652-788 from the human protein was used as the immunogen for the ACE antibody. |
Dilution range | Western blot: 1-2ug/ml,ELISA: order Ab without BSA for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This ACE antibody is available for research use only. |
UniProt ID | P12821 |
Storage | Store the ACE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the ACE antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE analysis of purified, BSA-free ACE antibody (clone ACE/3765) as confirmation of integrity and purity.
IHC staining of FFPE human small intestine with ACE antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human small intestine with ACE antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human kidney lysate with ACE antibody. Expected molecular weight 140-170+ kDa depending on glycosylation level.
ELISA, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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