ACAT1 Antibody

• Catalog Number: orb1263144
• Category: Antibodies
• Description: ACAT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Monkey
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: ACAT1
• UniProt ID: P24752
• NCBI: P24752
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Acetyl-CoA acetyltransferase, mitochondrial, Aceto
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: mouse liver lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of anti-ACAT1 Pab in SK-BR-3 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.