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Catalog Number | orb1259430 |
---|---|
Category | Antibodies |
Description | ACACA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human ACACA |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 240kDa |
Target | ACACA |
UniProt ID | Q13085 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Acetyl-CoA carboxylase 1, ACC1, ACC-alpha, Biotin Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ACACA antibody (orb1259430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of 293T cells using ACACA antibody (orb1259430) at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ACACA antibody (orb1259430) at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ACACA antibody (orb1259430) at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37 ℃ for 15 minutes after serum-starvation overnight (left). Blue: DAPI for nuclear staining.
FC, IHC-P | |
Bovine, Canine, Equine, Gallus, Porcine | |
Human, Mouse, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Monkey, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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