AC133 (CD133) Antibody (C-term)

• Catalog Number: orb1931899
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB1784
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, IF: 1:10~50, WB: 1:2000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 97202 Da
• Target: This AC133 (CD133) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AC133.
• UniProt ID: O43490
• NCBI: NP_001139322.1, NP_001139321.1, NP_001139319.1, NP_001139320.1, NP_001139324.1, NP_001139323.1, NP_006008.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Prominin-1, Antigen AC133, Prominin-like protein 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of CEM cells using AC133 (CD133) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of anti-AC133 (CD133) Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AC133 (CD133) antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence data is of Bone Marrow Mononuclear Cells stained with polyclonal CD133.

All lanes: Anti-hAC133-K848 at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.