ABO Antibody (Blood Group Antigen B)

• Catalog Number: orb749542
• Category: Antibodies
• Description: The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using > 5.000 samples of blood. The mAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This mAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HEB-20
• Tested applications: IF, IH
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B was used as the immunogen for the ABO antibody.
• Dilution range: Immunofluorescence: 2-4ug/ml,Immunohistology (formalin-fixed): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This ABO antibody is available for research use only.
• UniProt ID: P16442
• Storage: Store the ABO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group B antibody (HEB-20).

SDS-PAGE Analysis of Purified, BSA-Free ABO Antibody (clone HEB-20). Confirmation of Integrity and Purity of the Antibody.