ABL1/2 (pY393/439) antibody

• Catalog Number: orb665650
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ABL1/2 (Phospho-Y393/439)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393/439 of human ABL1/2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ABL1; ABL2
• Entrez: 11350, 27, 25
• UniProt ID: P00519, P42684, P00520, Q4JIM5
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-ABL1 antibody, anti-ABL antibody, anti-JTK7 a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ABL1/2 (Phospho-Y393/439) expression in AML12 (A), HCT116 (B), LO2 (C) whole cell lysates. (Predicted band size: 122; 128 kD; Observed band size: 210 kD)

Immunohistochemical analysis of ABL1/2 (Phospho-Y393/439) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.