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Catalog Number | orb420348 |
---|---|
Category | Antibodies |
Description | Abi1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to near c-terminal of the mouse Abi-1 protein. |
Concentration | 1.07 mg/mL |
Dilution range | ELISA: 5.0 µg/ml, WB: 1.0 ug/ml |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Abi1 is directed against mouse Abi1. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest cross reactivity with this protein in human and rat based on 100% homology for the immunogen sequence. Reactivity against homologues from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | Q8CBW3 |
NCBI | NP_001070658.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Abi1 Antibody, Abi-1, Abi 1, Abl Inter Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 65 kDa in size corresponding to Abi1 by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Abi1 antibody. Lane 1: Purified recombinant His-tagged Abi1 Isoform 2. Lane 2: Purified recombinant His-tagged Abi1 Isoform 3. Load: 2 µL per lane. Primary antibody: Abi1 antibody at 1:1000 for 2 hrs at RT. Secondary antibody: HRP conjugated goat-α-rabbit at 1:10000 for 60 min at RT. Blocking Buffer: 5% non-fat milk in TBS-t overnight at 4°C. Predicted/Observed size: 65 kDa for Abi1.
Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder. Lane 1: Abi1 recombinant protein. Load: 50 ng per lane. Primary antibody: Abi1 antibody at 1:1000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 30 min at RT. Blocking Buffer: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.
Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder. Lane 1: MEF3 KO. Lane 2: MEF3 WT. Lane 3: Mouse brain (p/n orb348715). Lane 4: Rat brain (p/n orb348727). Lane 5: HCT-116 WCL (p/n orb348712). Lane 6: HeLa WCL (p/n orb348668). Lane 7: NIH/3T3 WCL (p/n orb348714). Load: 35 µg per lane. Primary antibody: Abi1 antibody at 1:1000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 30 min at RT. Blocking Buffer: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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