Abi1 antibody

• Catalog Number: orb420347
• Category: Antibodies
• Description: Abi1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to near c-terminal of the mouse Abi-1 protein.
• Concentration: 1.07 mg/ml
• Dilution range: ELISA: 5.0 µg/ml, WB: 1.0 ug/ml
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Abi1 is directed against mouse Abi1. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest cross reactivity with this protein in human and rat based on 100% homology for the immunogen sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q8CBW3
• NCBI: NP_001070658.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Abi1 Antibody, Abi 1, Abi-1, Abl Inter
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 65 kDa in size corresponding to Abi1 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Abi1 antibody. Lane 1: Purified recombinant His-tagged Abi1 Isoform 2. Lane 2: Purified recombinant His-tagged Abi1 Isoform 3. Load: 2 µL per lane. Primary antibody: Abi1 antibody at 1:1000 for 2 hrs at RT. Secondary antibody: HRP conjugated goat-α-rabbit at 1:10000 for 60 min at RT. Blocking Buffer: 5% non-fat milk in TBS-t overnight at 4°C. Predicted/Observed size: 65 kDa for Abi1.

Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder. Lane 1: Abi1 recombinant protein. Load: 50 ng per lane. Primary antibody: Abi1 antibody at 1:1000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 30 min at RT. Blocking Buffer: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.

Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder. Lane 1: MEF3 KO. Lane 2: MEF3 WT. Lane 3: Mouse brain (p/n orb348715). Lane 4: Rat brain (p/n orb348727). Lane 5: HCT-116 WCL (p/n orb348712). Lane 6: HeLa WCL (p/n orb348668). Lane 7: NIH/3T3 WCL (p/n orb348714). Load: 35 µg per lane. Primary antibody: Abi1 antibody at 1:1000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 30 min at RT. Blocking Buffer: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.