ABCG2 rabbit pAb

• Catalog Number: orb764444
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ABCG2
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ABCG2. AA range:289-338
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 72
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-ABCG2 antibody, anti-ABCP antibody, anti-BCRP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.

Western Blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 (diluted at 1:5000, 25°C, 1 hour).

Western Blot analysis of various cells using ABCG2 Polyclonal Antibody.

Western Blot analysis of HT29 cells using ABCG2 Polyclonal Antibody.