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Catalog Number | orb1935370 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB13782 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 72314 Da |
Target | This ABCG2 (BCRP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-335 amino acids from the Central region of human ABCG2 (BCRP). |
UniProt ID | Q9UNQ0 |
NCBI | NP_001244315.1, NP_004818.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | ATP-binding cassette sub-family G member 2, Breast Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ABCG2 Antibody (Center) in 293 cell line lysates (35 ug/lane). ABCG2 (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of ABCG2 (BCRP) Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ABCG2 (BCRP) Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ABCG2 (BCRP) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ABCG2 (BCRP) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.