ABCA2 Antibody

• Catalog Number: orb1246599
• Category: Antibodies
• Description: ABCA2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KKQSDNLEQQETEP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to .NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ABCA2
• UniProt ID: Q9BZC7
• NCBI: NP_997698.1, NP_001597.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ABCA2, ATP-binding cassette, sub-family A (ABC1),
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to .NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246599 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.