6X HIS EPITOPE TAG antibody

• Catalog Number: orb345394
• Category: Antibodies
• Description: 6X HIS EPITOPE TAG antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Isotype: IgG
• Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
• Concentration: 1.22 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:40,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-6X His Epitope Tag Antibody, anti-HIS,
• Note: For research use only
• Application notes: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
• Expiration Date: 12 months from date of receipt.

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.

Western blot of Rabbit Anti-6xHIS Epitope Tag antibody. Lane: NFR1-HIS recombinant protein. Load: 50 ng per lane. Primary antibody - 1: NRF1 antibody at 1:1000 overnight at 4°C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.