67kDa Laminin Receptor/RPSA Antibody

• Catalog Number: orb381096
• Category: Antibodies
• Description: 67kDa Laminin Receptor/RPSA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Hamster, Monkey
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human RPSA recombinant protein (Position: S2-S138). Human RPSA shares 100% and 99.3% amino acid (aa) sequence identity with mouse and rat RPSA, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 32854 MW
• UniProt ID: P08865
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: 40S ribosomal protein SA ;37 kDa laminin receptor
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-RPSA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-RPSA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of RPSA using anti-RPSA antibody.Lane 1:HELA cell; 2:U2OS cell.

IF analysis of RPSA using anti-RPSA antibody.RPSA was detected in immunocytochemical section of U20S cells.

IHC analysis of RPSA using anti-RPSA antibody.RPSA was detected in paraffin-embedded section of human mammary cancer tissue.