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Catalog Number | orb1129226 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to 58K Golgi protein(Internal) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Synthetic peptide derived from Human 58K Golgi protein(Internal). |
Concentration | 100ug/100ul |
Dilution range | Recommended dilutions WB: 1/500~2000 Predicted molecular weight: 59 kDa. Observed band size : 120 kDa (Why is the actual band size different from the predicted? Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.) Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Buffer/Preservatives | PBS, pH 7.4 with 0.02% sodium azide. |
Note | For research use only |
Application notes | Recommended dilutions WB: 1/500~2000 Predicted molecular weight: 59 kDa. Observed band size : 120 kDa (Why is the actual band size different from the predicted? Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.) Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Expiration Date | 12 months from date of receipt. |
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