5-DBCO-PEG4-dUTP

• Catalog Number: orb532299
• Category: Tools
• Description: 5-DBCO-PEG 4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins.
• Concentration: 10 mM-11 mM
• Form/Appearance: solution in 100 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1057.83 g/mol (free acid); Detected MW: 1057.25 g/mol (free acid)
• SMILES: c1(=O)[nH]c(=O)n(cc1/C=C/CNC(=O)CCOCCOCCOCCOCCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
• Formula: C₄₂H₅₄N₅O₂₁P₃
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. ; Spectroscopic Propertie: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Expiration Date: 12 months from date of receipt.