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Catalog Number | orb1231345 |
---|---|
Category | Proteins |
Description | 4-1BB / TNFRSF9 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His Tag |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 18.8 kDa |
Target | TNFRSF9 |
Protein Sequence | Val 29 - Ile 191 |
Source | HEK293 cells |
NCBI | XP_008264195.1 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | TNFRSF9,4-1BB,CD137,CDw137,ILA Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.8 kDa. The protein migrates as 27-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Rabbit 4-1BB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human 4-1BB Ligand, Fc Tag at 2 ug/ml (100 uL/well) can bind Rabbit 4-1BB, His Tag with a linear range of 1-39 ng/mL.
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
44.2 kDa | |
HEK293 cells |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
19.2 kDa | |
HEK293 cells |
WB | |
> 90% as determined by SDS-PAGE. | |
19.5 kDa | |
HEK293 cells |