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Catalog Number | orb1231189 |
---|---|
Category | Proteins |
Description | 4-1BB / TNFRSF9 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Mouse IgG Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 44.2 kDa |
Target | TNFRSF9 |
UniProt ID | Q07011 |
Protein Sequence | Leu 24 - Gln 186 |
Source | HEK293 cells |
NCBI | NP_001552.2 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, pH 7.5 |
Alternative names | TNFRSF9,4-1BB,CD137,CDw137,ILA Read more... |
Note | For research use only |
Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 44.2 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human 4-1BB Ligand, Fc Tag at 1 ug/ml (100 uL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.6-5 ng/mL.
Immobilized Human 4-1BB Ligand, His Tag at 2 ug/ml (100 uL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.1-4 ng/mL.
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
19.2 kDa | |
HEK293 cells |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
18.8 kDa | |
HEK293 cells |
WB | |
> 90% as determined by SDS-PAGE. | |
19.5 kDa | |
HEK293 cells |