You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1946759 |
---|---|
Category | Assays and Kits |
Description | 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
Range | 3.13-200 ng/mL |
Target | 25-OH-D |
Sensitivity | 0.91 ng/mL |
Alternative names | 25 Hydroxy Vitamin D; Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25 Hydroxy Vitamin D(25-OH-D) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25 Hydroxy Vitamin D(25-OH-D). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25 Hydroxy Vitamin D(25-OH-D) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
All | |
3.13-200 ng/mL | |
0.91 ng/mL |
Rat | |
78.13-5000 ng/mL | |
7.7 ng/mL |
Mouse | |
78.13-5000 ng/mL | |
7.7 ng/mL |
Human | |
78.13-5000 ng/mL | |
7.7 ng/mL |
Filter by Rating