14-3-3 zeta+delta Rabbit Polyclonal Antibody

• Catalog Number: orb100007
• Category: Antibodies
• Description: 14-3-3 zeta+delta Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta (51-150/245aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: YWHAZ
• UniProt ID: P63104
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 14 3 3 protein delta; 14 3 3 protein zeta/delta; 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (grenn line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.

Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.