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Human VEGF165 Protein
Catalog Number: orb257923
| Catalog Number | orb257923 |
|---|---|
| Category | Proteins |
| Description | Human VEGF165, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
| Reactivity | Human |
| Tag | Native |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 19.2 kDa |
| Target | VEGF165 |
| UniProt ID | P15692 |
| Source | Human VEGF165, premium grade (orb257923) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
| Expression System | HEK293 |
| Biological Activity | Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Ala 27 - Arg 191 |
| Endotoxins | 0.01 EU per μg |
| NCBI | NP_001165097.1 |
| Storage | -20°C to -70°C for 24 months in lyophilized state; -70°C for 24 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF Read more... |
| Note | For research use only |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 19.2 kDa. As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE). |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human VEGF protein
Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations
Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations
Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. orb257923) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
- Item 1 of 3
Human VEGF165 Protein [orb257924]
Unconjugated
90%
21.1 kDa
Human VEGF165, His Tag (orb257924) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
1 mg, 50 μg 
Human VEGF165 Protein [orb1289549]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgRecombinant Human VEGFA Protein, C-His [orb1088542]
CLIA, EIA, LF
> 90% as determined by SDS-PAGE
20.8 kDa
HEK293
1 mgRecombinant Human VEGF165 Protein [orb1550376]
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC
KMP2113, Recombinant Human VEGF165 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ala27-Arg191) of Human VEGF165 fused with no tag .
100 μg, 500 μgBiotinylated Recombinant Human VEGF165 Protein, Biotinylated [orb1550377]
> 95% by Tris-Bis PAGE
KMP2112, Biotinylated Recombinant Human VEGF165 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ala27-Arg191) of Human VEGF165 fused with a His Tag and Avi tag at the C-terminal.
100 μg, 500 μg
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