Human TNF-alpha Protein

• Catalog Number: orb257883
• Category: Proteins
• Description: Human TNF-alpha, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 17.4 kDa
• Target: TNF-alpha
• UniProt ID: P01375
• Source: Human TNF-alpha, premium grade (orb257883) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1).
• Expression System: HEK293
• Biological Activity: Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Val 77 - Leu 233
• Endotoxins: 0.02 EU per μg
• NCBI: NP_000585.2
• Storage: -20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human TNF-alpha protein

Line graph illustrates about the sensitivity range of Human TNF-alpha protein using different concentrations

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. orb257883) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).