Human IGF-I Protein

• Catalog Number: orb1146986
• Category: Proteins
• Description: Human IGF-I, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05019-1).
• Reactivity: Human
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 9.5 kDa
• Target: IGF-I
• UniProt ID: P05019
• Source: Human IGF-I, His Tag, premium grade (orb1146986) is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05019-1).
• Expression System: HEK293
• Biological Activity: Biotinylated Human IGF-I R, His, immobilized on CM5 Chip can bind Human IGF-I, His Tag, premium grade with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).
• Expression Region: Gly 49 - Ala 118
• Endotoxins: 0.1 EU per μg
• NCBI: NP_001104755.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGF-I,IGF1A,somatomedin C,MGF
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 9.5 kDa. The protein migrates as 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Biotinylated Human IGF-I R, His, Avitag immobilized on CM5 Chip can bind Human IGF-I, His Tag, premium grade (Cat. No. orb1146986) with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).