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Catalog Number | orb735041 |
---|---|
Category | Proteins |
Description | Human IFN-alpha 1, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 21.3 kDa |
Target | IFN-alpha 1 |
UniProt ID | P01562 |
Source | Human IFN-alpha 1, His Tag (orb735041) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1). |
Expression System | HEK293 |
Biological Activity | Loaded Human IFN-alpha 1, His Tag on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Cys 24 - Glu 189 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Human IFN-alpha 1, His Tag (Cat. No. orb735041) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. orb257558) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
> 97% as determined by SDS-PAGE and HPLC. | |
19.5 kDa | |
E.Coli |
> 96% as determined by SDS-PAGE and HPLC. | |
19.5 kDa | |
E.Coli |
Unconjugated | |
90% | |
73.8 kDa | |
Human IFNAR1, Fc Tag (orb257558) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # NP_000620). |
Unconjugated | |
95% | |
45.8 kDa | |
Human IFN-alpha 1, Fc Tag (orb1147003) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1). |
Unconjugated | |
95% | |
49.0 kDa | |
Human IFN-alpha / beta R1 Protein, His Tag (orb867302) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # P17181-1). |
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