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Catalog Number | orb762429 |
---|---|
Category | Proteins |
Description | Human EGF Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1). |
Reactivity | Human |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 8.3 kDa |
Target | EGF |
UniProt ID | P01133 |
Source | Human EGF Protein, His Tag, premium grade (orb762429) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1). |
Expression System | HEK293 |
Biological Activity | Immobilized Human EGF Protein, His Tag, premium grade at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag with a linear range of 0.2-5 ng/mL (QC tested). |
Expression Region | Asn 971 - Arg 1023 |
Endotoxins | 0.1 EU per μg |
NCBI | NP_001954.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | EGF,URG,HOMG4 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human EGF Protein, His Tag, premium grade (Cat. No. orb762429) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. orb257416) with a linear range of 0.2-5 ng/mL (QC tested).
Unconjugated | |
95% | |
70.5 kDa | |
Human EGF R, His Tag (orb257415) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). |
> 97% as determined by SDS-PAGE and HPLC. | |
9.7 kDa | |
E.Coli |
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